Cell Culture Media And Methods For Generating Human Alveolar Macrophage-like Cells


Provided here methods of generating human alveolar macrophage-like cells in-vitro from blood-derived monocytes by culturing them in a cell culture medium containing a mixture of a surfactant and two or more cytokines. This mixture can contain calfactant, granulocyte-macrophage colony-stimulating factor, transforming growth factor beta, and interleukin-10.

Application Number

17657344 (non-provisional)

Date of Application

March 30, 2022


Larry S. Schlesinger and Susanta Pahari